SIGMA| 人血清白蛋白

貨號:

A9511

品牌:

Sigma

規格:

100MG,1G,10G

價(jià)格:

詢(xún)價(jià)

概述

人血清白蛋白第五組分，HSA,

A9511-100MG
A9511-500MG
A9511-1G
A9511-5G
A9511-10G
A9511-25G

研究領(lǐng)域:

細胞生物學(xué)

詳述

ALBUMIN, HUMAN

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The source material for all human albumins made by Sigma has tested negative for HIV and HBSAG.

Sigma tests the solubility of human albumin powders in water at 50 mg/. In the experience of Sigma production chemists, aqueous aliquots stored at –20 °C are stable for several months. Repeated freezing and thawing of solutions is not recommended.

A9511	Albumin from human serum, lyophilized powder, ≥97% (agarose gel electrophoresis),	H₂O: soluble50?mg/mL	≥97% (agarose gel electrophoresis)	Sigma	?
A1653	Albumin from human serum, lyophilized powder, ≥96% (agarose gel electrophoresis),	H₂O: soluble50?mg/mL	≥96% (agarose gel electrophoresis)	Sigma	?
A3782	Albumin from human serum, lyophilized powder, Fatty acid free, Globulin free, ≥99% (agarose gel electrophoresis),	H₂O: soluble50?mg/mL	≥99% (agarose gel electrophoresis)	Sigma	?
A8763	Albumin from human serum, lyophilized powder, essentially globulin free, ≥99% (agarose gel electrophoresis),	H₂O: soluble20?mg/mL	≥99% (agarose gel electrophoresis)	Sigma	?
A1887	Albumin from human serum, lyophilized powder, essentially fatty acid free,	H₂O: soluble50?mg/mL, clear to slightly hazy, yellow	?	Sigma	?
A4327	Albumin from human serum, lyophilized powder, essentially protease free, ≥96% (agarose gel electrophoresis),	H₂O: soluble50?mg/mL	≥96% (agarose gel electrophoresis)	Sigma	?
A5843	Albumin from human serum, lyophilized powder, low endotoxin,	H₂O: soluble50?mg/mL	?	Sigma	?
05420	Albumin from human serum, lyophilized powder, ≥95.0% (agarose gel electrophoresis),	?	≥95.0% (agarose gel electrophoresis)	Sigma ?

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PRODUCT NO.

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PREPARATION/PURIFICATION

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SPECIAL CHARACTERISTICS

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A 1653

Fraction V1

From human serum.

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Powder

96-99% by agarose gel (remainder mostly globulins)

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A 2817

Fraction V1

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Powder

96-99% by agarose gel (remainder mostly globulins)

Produced specifically for bulk usage.

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A 1887

Fraction V1, heat step2 Prepared from A 1653.

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Powder

396% by agarose gel

Essentially fatty acid free (～0.005%)

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A 9511

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1× crystallized and lyophilized.

Prepared from A 1653 using method IV of Cohn, et al.3

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Powder

97-99% by agarose gel

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A 8763

Prepared from A 1653.4

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Powder

Essentially globulin free

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A 3782

Prepared from A 8763.4

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Powder

Essentially fatty acid free (～0.005%) Essentially globulin free

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A 4327

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Prepared from A 1653.

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Powder

396% by agarose gel

Essentially protease free (<0.0001 u/mg protein) Essentially alkaline phosphatase free (0.001 u/mg protein) Essentially peroxidase free (<0.001 u/mg protein)

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A 3173

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Prepared from A 2817.

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Powder

Low endotoxin content (20.1 ng/mg protein)

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A 6784

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Contains approx. 0.85% NaCl. Contains 0.05% NaN3 as preservative. Aseptically filled.

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10% Solution

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A 6909

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Contains approx. 0.85% NaCl. Contains 0.05% NaN3 as preservative. Aseptically filled.

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30% Solution

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A 9080

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From human plasma. Contains approx. 0.85% NaCl. Aseptically filled.

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30% Solution Protease free.

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A 2830

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From human serum.

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Solution in 40 mM potassium phosphate, pH 7.0

14C-Methylated

5-50 ìCi per mg protein

CAS NUMBER: 70024-90-7

MOLECULAR WEIGHT: 66,248.35 or 66,4378?(based on amino acid composition)

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STRUCTURE:5

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The amino acid sequence and structure of human albumin have been determined. Human albumin is a protein with no carbohydrate content. It is a single polypeptide chain with one free sulfhydryl group on residue # 34 and 17 intrachain disulfide bonds.

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Amino Acid????? Asp Asn Thr Ser Glu Gln Pro Gly Ala Cys Val Met Ile Leu Tyr Phe His Lys Trp Arg

Residues???????? 39??? 15?? 30?? 22? 60?? 23 ?25 ? PHYSICAL ?PROPERTIES:5	12 63	35 ?39?? 6? ?8? 61 18???? 30? 16 ?58 ?1????????????? 23
Sedimentation constant, S20,W? X ?1013	?	4.6 (monomer), 6.5 (dimer)
Diffusion constant, D20,W? X 107	?	6.1
Partial specific volume, V20	?	0.733
Intrinsic viscosity, ?	?	0.042
Frictional ratio, f/f0	?	1.28
Overall dimensions, ?	?	38 X 150
Isoelectric point (?/2 = 0.15)	?	4.7
Isoionic point (?/2 = 0)	?	5.2
Electrophoretic mobility, pH 8.6, ?/2 = 0.15	?	-5.9
Refractive index increment (578 nm) X?? 10-3	?	1.89
Optical absorbance, A279 nm? (1 ?gram/liter)	?	0.531
Mean residue rotation, ?[m']233	8590	?
Mean residue ellipticity	?	17 [è]209 nm; 16 [è]222 nm
Estimated á-helix, %	?	48
Estimated a-form, %	?	15
SOLUBILITY / SOLUTION STABILITY:	?	?

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儲存與安全

儲存:

2-8℃

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SIGMA| 人血清白蛋白