Application

The kit contains all essential reagents for the detection of membrane-blotted, digoxigenin-labeled nucleic acid hybrids. It can be used in a variety of hybridization techniques:??Southern blots
??Northern blots
??Other nucleic acid blotting applications
??In situ?hybridization applications

General description

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Detection of digoxigenin-labeled nucleic acids by enzyme immunoassay and enzyme-catalyzed color reaction.

Sigma Life Science is committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical.? The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Packaging

1 kit containing 5 components.

Principle

DIG-labeled nucleic acids are detected after hybridization in an enzyme-linked immunoassay with a highly specific anti-DIG-AP antibody conjugate and the color substrates NBT and BCIP.

Preparation Note

Working concentration:?Flow cytometry: 2 μg/ml (0.2 μg/100 μl/106 cells); Immunohistocytochemistry: 6 μg/ml
Working concentration of conjugate depends on application and substrate.
Storage conditions (working solution):???Anti-Digoxigenin-AP Conjugate (vial 3): 2 to 8 °C, it is stable for 12 months at this temperature
??NBT/BCIP (vial 4): 2 to 8 °C, stable
Note:?During shipment of the kit on dry ice, a precipitate may occur which is dissolved by briefly warming to 37 °C
??The blocking reagent (vial 5) is stable for 36 months and can be stored dry at 4 to 8 °C.
??Autoclaved stock solution can be stored for several days to a week either unopened at 15 to 25 °C or at 4 to 8 °C after opening. Alternatively, it can be stored in aliquots at -15 to -25 °C for up to 6 months.